Figure 8B – Vps13-dependent membrane structure is closely associated with the plasma membrane
of nurse cells

Large scale EM of the selected egg chamber and region containing nurse cells and follicle cells. Artificial colors were used to indicate engulfing follicle cells (green), nurse cell cytoplasm (in magenta), nurse cell nucleus (in blue), translucent vacuoles (in yellow) and vesicles representative for the degradative machinery (in orange). B’. The magenta line indicates the sites of close contact between the plasma membrane of the nurse cell and the plasma membrane of the follicle cell (NC/FCpm), the blue line indicates the nuclear envelope (NE). NC Cyto=cytoplasm of nurse cells, FC Cyto= cytoplasm of stretch follicle cells, VDP=vesicles containing cellular degradation products, TV’s=translucent vacuoles.

Figure 9A - Membrane structure is absent in Vps13 mutants

Large scale EM of the selected region, containing nurse cells and follicle cells See Figure 8 above for details.

Figure S12 - Absence of membrane structure associated with the plasma membrane in Vps13 mutants

Ultrastructural analysis (as in Figure 8, 9) of egg chambers of the Vps13PB mutant. A. Large scale EM of the selected region, containing nurse cells and follicle cells. Artificial colors were used to indicate engulfing follicle cells (green), nurse cell
cytoplasm (in light magenta), nurse cell nucleus (in blue), translucent vacuoles (in yellow) and vesicles representative for the degradative machinery (in orange). A’. Schematic containing structures visible in A. The magenta line indicates the sites of close contact between the plasma membrane of the nurse cell and the plasma membrane of the follicle cell (NC/FCpm), the yellow line indicates the nuclear envelope (NE). NC Cyto=cytoplasm of nurse cells, FC Cyto= cytoplasm of stretch follicle cells, VDP=vesicles representing degradative machinery, TV’s=translucent vacuoles.