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Nanotomy: Large-scale electron microscopy (EM) datasets

Anatomy at the nanoscale: nano-anatomy or nanotomy

NEW: EDX-based ColorEM in life sciences EDX-based ColorEM in life sciences Golddigger (ImageJ)
Scotuzzi/ Kuipers/ Wensveen, de Boer, Hagen, Hoogenboom/ Giepmans (2017). Scientific Reports 7:45970. (Pdf)

Sjollema & Giepmans (2016) Semi-Automatic analyzing gold and QDs. Imaging & Microscopy Oct. 24 2016.

Mix and match with FLIPPERs Nanotomy workshops
Kuipers J (...) Giepmans BN (2015) FLIPPER, a probe for CLEM, allows identification and quantitative analysis of various cells and organelles. Cell & Tissue Research 360:61 Nanotomy to teach students (BSc, MSc, PhD) structure/ function relationships in biomedicine, which is also implemented at other universities.
  All published nanotomy (Year/author) Blistering skin & mucosa Islet research related to nPOD
Sokol E (...) Giepmans BN (2015) Large-Scale EM Maps of Patient Skin and Mucosa Provide Insight into Pathogenesis of Blistering Diseases. J. Invest. Dermatol.135:1763 Type 1 diabetes (T1D) is caused by destruction of insulin producing beta cells. JDRF developed nPOD. This biobank is shared worldwide to understand T1D and help to prevent or cure this disease [restricted ].
(S)(T)EM contrast & Quantum dots Islets in Type 1 Diabetes (rat)
Kuipers J / de Boer P & Giepmans BN (2015) Scanning EM of non-heavy metal stained biosamples: Large-field of view, high contrast and highly efficient immunolabeling. Exp. Cell Res.337:202 Ravelli RB (...) Giepmans BN (2013) Destruction of tissue, cells and organelles in type 1 diabetic rats presented at macromolecular resolution. Sci. Reports 3:1804

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