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Nanotomy: Large-scale electron microscopy (EM) datasets

Anatomy at the nanoscale: nano-anatomy or nanotomy

All published nanotomy (Year/author) EDX-based ColorEM in life sciences Macrostomum Lignano reproduction

All nanotomy datasets (table).

Scotuzzi/ Kuipers/ Wensveen, de Boer, Hagen, Hoogenboom/ Giepmans (2017). Scientific Reports 7:45970. (Pdf)

Grudniewska/ Mouton (...) Berezikov (preprint on BioRxiv).

Nanotomy procedure (zebrafish) Golddigger (ImageJ/ FIJI) Islet isolation and survival

Kuipers J (...) Giepmans BN (2016) Large-scale STEM of zebrafish brain. J. of Visualized Experiments doi: 10.3791/53635.

Sjollema & Giepmans (2016) Semi-Automatic analyzing gold and QDs. Imaging & Microscopy Oct. 24 2016. de Vos P (...) Faas MM (2016) Islet isolation impacst β-cells with reduced survival of rat islet-allografts. PLos ONE 11: e0147992
Salivary gland organoid Mix and match with FLIPPERs Islet research related to nPOD
Maimets M (...) Coppes RP (2016). Long-term in vitro expansion of salivary gland stem cells by Wnt Stem Cell Reports 6:150–162pdf Kuipers J (...) Giepmans BN (2015) FLIPPER, a probe for CLEM, allows analysis of cells and organelles. Cell & Tissue Research 360:61 Type 1 diabetes is caused by destruction of insulin producing beta cells. JDRF financed the nPOD biobank to understand T1D and help to prevent or cure this disease [restricted].
G4 in heterochromatin Islets in Type 1 Diabetes (T1D; rat) Nanotomy T1D workshops and more
Hoffmann RF (...) Lansdorp PM (2016) Guanine quadruplex structures localize to heterochromatin. Nucleic Acid Research 44:152 Ravelli RB (...) Giepmans BN (2013) Destruction of cells and organelles in T1D rats at macromolecular resolution. Sci. Reports 3:1804 To teach structure/ function in biomedicine (BSc, MSc, PhD), also at other universities, including the combination with CLEM and histology.
Blistering skin & mucosa (S)(T)EM contrast & Quantum dots Links to other EM data bases
Sokol E (...) Giepmans BN (2015). Patient skin and mucosa provide Insight into blistering diseases. J. Invest. Dermatol.135:1763 Kuipers J / de Boer P & Giepmans BN (2015) Scanning EM of non-stained biosamples: High contrast and efficient immunolabeling. Exp. Cell Res.337:202 EMPIAR, CIL, etc.

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