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Nanotomy: Large-scale electron microscopy (EM) datasets

All published nanotomy (Year/author) Salivary gland organoid Islet isolation and survival

Papers and datasets with all nanotomy featured in a table.

Maimets M (...) Coppes RP (2016) Long-term in vitro expansion of salivary gland stem cells driven by Wnt signals Stem Cell Reports 6:150–162

de Vos P (...) Faas MM (2016) Enzymes for islet isolation impact chemokine-production and polarization of β-cells with reduced functional survival of immunoisolated rat islet-allografts. PLos ONE 11: e0147992

Nanotomy procedure (zebrafish) G4 in heterochromatin Islet research related to nPOD

Kuipers J (...) Giepmans BN (2016) Large-scale scanning transmission EM (nanotomy) of healthy and injured zebrafish brain. J. of Visualized Experiments doi: 10.3791/53635.

Hoffmann RF (...) Lansdorp PM (2016) Guanine quadruplex structures localize to heterochromatin. Nucleic Acid Research 44:152
Type 1 diabetes (T1D) is caused by destruction of insulin producing beta cells. JDRF developed nPOD. This biobank is shared worldwide to understand T1D and help to prevent or cure this disease [restricted ].
Mix and match with FLIPPERs Blistering skin & mucosa Introduction to nanotomy
Kuipers J (...) Giepmans BN (2015) FLIPPER, a probe for CLEM, allows identification and quantitative analysis of various cells and organelles. Cell & Tissue Research 360:61 Sokol E (...) Giepmans BN (2015) Large-Scale EM Maps of Patient Skin and Mucosa Provide Insight into Pathogenesis of Blistering Diseases. J. Invest. Dermatol.135:1763 This movie illustrates the steps taken from tissue to nanotomy.
(S)(T)EM contrast & Quantum dots Rac2 & mitochondrial dysfunction Nanotomy T1D workshops and more
Kuipers J / de Boer P & Giepmans BN (2015) Scanning EM of non-heavy metal stained biosamples: Large-field of view, high contrast and highly efficient immunolabeling. Exp. Cell Res.337:202 Capala ME (...) Schuringa JJ (2015) Mitochondrial Dysfunction in Human Leukemic Stem/Progenitor Cells upon Loss of RAC2. PLoS ONE 10:e0128585 We use Nanotomy to teach our students (BSc, MSc, PhD) structure/ function relationships in biomedicine, which is also implemented at other universities, including the combination with CLEM or combined with histology.
Neuroinflammation (zebrafish) Islets in Type 1 Diabetes (rat) Pemphigus Pathogenesis by E. Sokol
van Ham TJ (...) Giepmans BN (2014) Intravital correlated microscopy reveals differential macrophage and microglial dynamics during resolution of neuroinflammation. Dis. Models & Mech. 7:857 Ravelli RB (...) Giepmans BN (2013) Destruction of tissue, cells and organelles in type 1 diabetic rats presented at macromolecular resolution. Sci. Reports 3:1804 Sokol, E (2016) Pemphigus pathogenesis: insights from light and electron microscopy studies. Thesis, UMC Groningen, The Netherlands [restricted access]

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